A mad yearning for solitude: Timon the Misanthrope and his relevance to the study of ancient psychopathology.

Ancient Greek and Latin medical authors considered a flight into solitude a compelling sign of mental disturbance, frequently described as misanthropia, a word fraught with meaning beyond the medical discourse. The fictionalised character Timon of Athens, the quintessential misanthrope, can shed light on ancient cultural concepts of self-imposed isolation from human contact. To cope with the sense of unease this deviant behaviour induced, misanthropia was explained as 'madness', ridiculed in various genres of humour, morally condemned in philosophy, and ultimately demonized in Christian cosmology. These various attempts at containment echo in the medical works of the age, making it impossible to comprehend the concept of misanthropia in ancient medicine without taking full account of the cultural context.

["Splendid Human Material"-Anthropometric Constitutional Research to Statistically Determine the Normal Human Body (1914-1922)]. / "Prächtiges Menschenmaterial" ­ Anthropometrische Konstitutionsforschung auf der Suche nach dem statistischen Normalkörper (1914­1922).

At the center of this work stands the anthropometric research program during World War I for studying constitutional medicine and the connected series of investigations by the medical internists Theodor Brugsch, Hermann Rautmann and Max Berliner, their advances in the statistics of variability as well as the subsequent debate in constitutional medicine and pathology on the definition of the physical norm.In order to create a data basis for the "normal" body in the study of constitutional medicine, a series of young German internists undertook comprehensive anthropometric studies in the context of World War I, thereby taking advantage of the opportunity offered them by war to conduct a series of examinations of soldiers, but without having first reflected on methods of measurement, comparison, and evaluation. At the same time, the concept of the "normal" body, then only vaguely formed, still needed to be critically expounded. However, this changed during the subsequent period and led not only to a stronger emphasis on methodology, rather also to greater competency in mathematical statistics and philosophical cogitation on the meaning of the "norm". In this way, constitutional medicine originated the potent medical norm debate of the early 1920s which still resonates in medical theory today. By this means the few years following the end of World War I not only represented a turning point for constitutional medicine regarding the reflective use of methodology, but also introduced a new orientation of their research questions: away from the "normal" body to individuality.

["On a strictly scientific basis." German constitutional medicine establishing itself, 1911­1921]. / "Auf strengster wissenschaftlicher Grundlage." Die Etablierungsphase der modernen Konstitutionslehre 1911 bis 1921.

In the years between 1911 and 1921, modern constitutional medicine established itself as an interdisciplinary research program in German-speaking countries. Untouched by later holistic interpretations and still far from the ,,crisis of medicine" of the late 1920s early constitutional medicine was very attractive due to its scientific self-characterisation. Thus, it became influential across the medical disciplines. This paper examines history and subject matter of German modern constitutional medicine in its first decade, starting in 1911, the year constitutional medicine was first publicly discussed by the Wiesbaden congress for internal medicine, including its development during World War I and closing with the first textbooks for medical students in 1921.

Kynanthropy: canine madness in Byzantine late antiquity.

Those afflicted bark like dogs, scramble on all fours and loiter around graveyards - canine madness, referred to as kynanthropy, was an illness concept in its own right in the medicine of late antiquity. At roughly the same time as the medical description produced by Aëtius of Amida, the Syrian chronicler John of Ephesus, also from Amida, reported an epidemic of dog-like madness sweeping his home town in ad 560. The symptoms are identical and both authors are from Amida - what is the connection between the two depictions? In addition to the history of the medical concept, the example of the canine madness of Amida and its cultural embedding allows us to contextualize and interpret the significance of dog-like behaviour for the people of the sixth century AD.

Battling demons with medical authority: werewolves, physicians and rationalization.

Werewolves and physicians experienced their closest contact in the context of early modern witch and werewolf trials. For medical critics of the trials, melancholic diseases served as reference points for medical explanations of both individual cases and werewolf beliefs in general. This paper attempts to construct a conceptual history of werewolf beliefs and their respective medical responses. After differentiating the relevant terms, pre-modern werewolf concepts and medical lycanthropy are introduced. The early modern controversy between medical and demonological explanations forms the main part of this study. The history of werewolves and their medical explanations is then traced through to present times. An important point of discussion is to what extent the physicians' engagements with werewolves can be characterized as rationalization.

Isolation, establishment, and characterization of ex vivo equine melanoma cell cultures.

Gray horses spontaneously develop metastatic melanomas that resemble human disease, and this is often accompanied with metastasis to other organs. Unlike in other species, the establishment of primary equine melanoma cultures that could be used to develop new therapeutic approaches has remained a major challenge. The purpose of the study was to develop a protocol for routine isolation and cultivation of primary equine melanocytes. Melanoma tissues were excised from 13 horses under local anesthesia, mainly from the perianal area. The melanoma cells were isolated from the melanoma tissue by serial enzymatic digestion using dispase and collagenase. Out of the 13 excised melanomas, cell cultures from eight melanomas were established, which corresponded to a success rate 62%. These cells showed different degrees of melanin pigmentation. Characterization of these cells using confocal microscopy, FACs analysis and western blotting showed that they expressed melanoma-associated antigens; Melan-A, MAGE-1, and MAGE-3, and PCNA expression was higher in fast-proliferating isolates. The protocol we developed and established proved successful for routine isolation and cultivation of primary equine melanoma cells. This method provided a large number of primary equine melanoma cells that could be used to study new therapeutic approaches for treatment of equine melanomas.

Usefulness of a commercial equine IgG test and serum protein concentration as indicators of failure of transfer of passive immunity in hospitalized foals.

Detection of failure of transfer of passive immunity (FTPI) is important in reducing morbidity and mortality in neonatal foals. We investigated the performance of a commercial equine IgG test (SNAP Foal IgG Test Kit) to diagnose FTPI in hospitalized foals. Furthermore, we evaluated the usefulness of serum total protein (STP) and serum globulin (SG) concentrations as indicators of FTPI. Serum IgG concentration was measured by means of the SNAP test and single radial immunodiffusion, and SG and STP concentrations were determined by means of a clinical chemistry analyzer. Subjects were 67 hospitalized foals <19 days old. The SNAP test was repeated on 37 samples from 29 foals, with identical results for 24 samples (kappa statistic, 0.64; 95% confidence interval [CI], 0.46-0.82). The sensitivity of the SNAP test to detect serum IgG concentration [IgG] < or =400 and < or =800 mg/dl was 90% (95% CI, 71-98%) and 95% (85-99%), respectively, and the specificity was 79% (71-82%) and 52% (39-57%), respectively. Sensitivity for detection of [IgG] < or =400 mg/dl was not affected (P > .05) by plasma fibrinogen concentration, sepsis score, or bacteremia. Specificity for detection of [IgG] < or = 800 mg/dl was lower (P < .05) in foals with sepsis score < or =11 (50% [31-60%] versus 100% [8-100%]) and bacteremia (25% [5-56%] versus 62% [45-62%]). Sensitivity and specificity of [STP] < or = 5.0 g/dl for [IgG] < or =800 mg/dl was 94% (83-99%) and 47% (30-56%), respectively. Performance of the SNAP test in hospitalized foals is impaired because of low specificity, but can have usefulness provided that the properties of the test and characteristics of the foal being examined are considered when interpreting the results. The STP and SG concentrations are poor sole indicators of FTPI in hospitalized foals, but may be useful adjunctive tests.